BSA 低脂肪酸，無蛋白酶，低內(nèi)毒素
貨號(hào)：BV1080-100g
規(guī)格：100克
價(jià)格：800元
貯存：4度貯存
運(yùn)輸：常溫運(yùn)輸
有效期：3年
牛血清白蛋白（Bovine Serum Albumin, BSA）是牛血清中的一種球蛋白，又稱第五組分（需要注意的是，第五組分并不是BSA的一種組份，而是Edwin J. Cohn教授早期從血液中通過特定的分離技術(shù)得到5種組份，其中第五種就是白蛋白），CAS號(hào)為9048-46-8，包含583個(gè)氨基酸殘基，分子量為 66.430 kDa，等電點(diǎn)為4.7。牛血清白蛋白在生化實(shí)驗(yàn)中有廣泛的應(yīng)用，例如在western blot中作為封閉試劑。血液中的白蛋白主要起維持滲透壓作用、PH緩沖作用、載體作用和營(yíng)養(yǎng)作用。在動(dòng)物細(xì)胞無血清培養(yǎng)中，添加白蛋白可起到生理和機(jī)械保護(hù)作用和載體作用。
低脂肪酸牛血清白蛋白產(chǎn)品及應(yīng)用
牛血清白蛋白(低脂肪酸)，或者稱為無脂肪酸牛血清白蛋白，或者稱為Fat Acid Free BSA，產(chǎn)品不含有或含有極微量的脂肪酸。常規(guī)方法純化的BSA難以有效去除其結(jié)合的脂肪酸。除了特別純化的BSA產(chǎn)品外，市售的普通BSA試劑含脂肪酸量的范圍一般是: 每mol BSA含0.2~1.5 mol脂肪酸。然而，一些生物醫(yī)學(xué)研究要求使用不含游離脂肪酸的BSA，否則BSA所含高濃度的脂肪酸將影響細(xì)胞培養(yǎng)、分化、代謝反應(yīng)、生化測(cè)定等。例如，在3T3-L1脂肪細(xì)胞分化過程中不能使用普通BSA試劑，否則影響分化和細(xì)胞代謝狀態(tài)；在測(cè)定培養(yǎng)的組織細(xì)胞釋放的游離脂肪酸濃度時(shí)，也必須用不含脂肪酸的BSA，否則難以準(zhǔn)確測(cè)定甘油三酯水解和游離脂肪酸釋放。
BSA低脂肪酸產(chǎn)品是使用專利的熱處理方法純化而來的超純粉末。經(jīng)過專門的去脂純化處理，脂肪酸含量低于0.05% (w/w)，性價(jià)比高，方便溶解。該無脂肪酸BSA可用于脂肪代謝相關(guān)分析的載體蛋白，也可與某種特異性的脂肪酸結(jié)合以形成白蛋白，可應(yīng)用于增強(qiáng)細(xì)胞培養(yǎng) 體系的性能；若實(shí)驗(yàn)中脂肪或脂肪酸的存在影響激素或者膽固醇的讀數(shù)時(shí)，這個(gè)產(chǎn)品是首選；還可以作為某些脂肪以及脂肪酸分析的替代品。
Bovine Serum Albumin
low FFA content, protease free,low endotoxin
General Description: Bovine
Serum Albumin (BSA) is the major protein species found in bovine blood plasma
and has applications in life science disciplines such as cell culture, in-vitro
diagnostics, human and veterinary pharmaceuticals, molecular biology, serology
and general research.
Bovogen Biologicals' BSA (Fraction V) is manufactured
using a proprietary heat shock process under GMP conditions. The BSA purity is consistently measured at 99 – 100%.
The BSA is a premium grade
product that is ideal for a variety of applications requiring a product with
high protein purity and low endotoxin. It is also fatty acid free,
IgG free, protease free and is bovine virus free.
Raw Material Origin: Manufactured from
the plasma of animals of 100% Australian origin that have undergone veterinary
ante- and post-mortem inspection and have been approved for human consumption.
Appearance: Pale-cream to white coloured, lyophilized
powder.
Applications:
-
general additive in growth
media for tissue and cell culture
-
protein standard
-
general research or
diagnostic assay reagent
-
assays requiring low
endotoxin levels
-
additive in specific
diluents
-
vaccine production
-
blocking agent in
immunoassays
-
stabiliser in certain types
of protein ligands
-
reagent for protease
sensitive immunoassays
-
hybridization
-
additive in serum reduced or
serum free media
-
additive in fatty acid sensitive
cell culture systems
-
additive to assist in
immunohaemaglutination
Storage and Handling: Product is shipped at ambient temperature however,
recommended storage is at 2-8°C.
引用BSA發(fā)表文章列表（6篇）：
1. [2014 IF=1.24] Water-soluble protein molecular weight istribution
and effects on wheat malt quality uring malting.
Journal: J. Inst. Brew. 2014; 120: 399–403
Authors: Lan Xie, Yuhong Jin, Jinhua Du and Kaili Zhang
Institution：College of
Food Science and Engineering, Shandong Agricultural University
Paper
link： https://doi.org/10.1002/jib.182
2. [2021 IF=6.95] Protein FT-IR amide bands are beneficial to
bacterial typing.
Journal: International Journal of Biological
Macromolecules 2022; 207: 358–364
Authors: Jinghang Xiea, Fan Yang, Haimei Shia, Jintao Yana, Hao Shena,
Shaoning Yua, Ning Gana, Bin Fenga, Li Wang.
Institution：School of
Material Science and Chemical Engineering, Ningbo University
Paper
link：https://linkinghub.elsevier.com/retrieve/pii/S0141813022004172
3. [2021 IF=7.96] Developing a novel Bifidobacterium phage
quantitative polymerase chain reaction-based assay for tracking untreated waste.
Journal: Science of The Total Environment Volume 838, Part 2, 10 September 2022, 155815
Authors: XiangLi, Warish Ahmed，Ziqi Wu，Yu Xia
Institution：Southern
University of Science and Technology
Paper
link：https://linkinghub.elsevier.com/retrieve/pii/S0048969722029126
4. [2022 IF=3.9] The effect of barley to wheat ratio inmalt blends on
protein composition and physicochemical characteristics of wort and beer.
Journal: J. Food Sci. 2023;88:193–203.
Authors: Xiu Li，Junhua Yang，Lan Xie，Yuhong Jin，Junhan Liu，Wenjiao Xing
Institution：Shandong Agricultural
University
Paper
link：https://ift.onlinelibrary.wiley.com/doi/abs/10.1111/1750-3841.16428
5. [2022 IF=5.3] Immunofuorescence staining of chloroplast
proteins with frozen sections of plant tissues.
Product:
BSA Cat:BA0332
Journal: Plant Cell Reports (2024) 43:168
Authors: Lulu Wang,Fang Zeng,Yongjuan Jiao,
Qingqing Zhou,Jinjie An,Hongbo Gao
Institution：College
of Biological Sciences and Technology, Beijing Forestry University
Paper
link：https://doi.org/10.1007/s00299-024-03255-2
6. [2023 IF=6] DRD2-Mediated AMPK Ubiquitination Regulates the Occurrence
of Hepatic Steatosis.
Product:
BSA Cat:BV1080
Journal: Liver International, 2025 45:e70053
Authors: Peng Ma, Hao Ou. Junze Cai, Yuanli Zhang, Yu Ou
Institution：School of
Life Science and Technology, China Pharmaceutical University
Paper
link：https://doi.org/10.1111/liv.70053